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( A ) Western blot detection of PD-1 protein expression; ( B ) In vitro bactericidal activity of Kupffer cells against K. pneumoniae evaluated by enumerating viable bacteria; ( C–E ) IL-6 ( C ), <t>IL-12</t> ( D ), and TNF-α ( E ) levels measured by ELISA; (F-H) Flow-cytometric detection of IL-6( F ), IL-12( G ), and TNF-α(H) proportions; ( I ) Flow-cytometric analysis of Kupffer-cell phagocytic capacity; ( J ) Western blot detection of SHP2, p-SHP2, STAT1, p-STAT1, NF-κB p65, and p-NF-κB p65. * P < 0.05.
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( A ) Western blot detection of PD-1 protein expression; ( B ) In vitro bactericidal activity of Kupffer cells against K. pneumoniae evaluated by enumerating viable bacteria; ( C–E ) IL-6 ( C ), IL-12 ( D ), and TNF-α ( E ) levels measured by ELISA; (F-H) Flow-cytometric detection of IL-6( F ), IL-12( G ), and TNF-α(H) proportions; ( I ) Flow-cytometric analysis of Kupffer-cell phagocytic capacity; ( J ) Western blot detection of SHP2, p-SHP2, STAT1, p-STAT1, NF-κB p65, and p-NF-κB p65. * P < 0.05.

Journal: Journal of Microbiology and Biotechnology

Article Title: Anti-PD-1 Therapy Alleviates Klebsiella pneumoniae -Induced Liver Abscess by Effectively Activating Kupffer Cells

doi: 10.4014/jmb.2602.02040

Figure Lengend Snippet: ( A ) Western blot detection of PD-1 protein expression; ( B ) In vitro bactericidal activity of Kupffer cells against K. pneumoniae evaluated by enumerating viable bacteria; ( C–E ) IL-6 ( C ), IL-12 ( D ), and TNF-α ( E ) levels measured by ELISA; (F-H) Flow-cytometric detection of IL-6( F ), IL-12( G ), and TNF-α(H) proportions; ( I ) Flow-cytometric analysis of Kupffer-cell phagocytic capacity; ( J ) Western blot detection of SHP2, p-SHP2, STAT1, p-STAT1, NF-κB p65, and p-NF-κB p65. * P < 0.05.

Article Snippet: The concentrations of TNF-α (E-EL-M3063, Elabscience, China), IL-6 (E-EL-M0044, Elabscience, China), and IL-12 (E-EL-M3062, Elabscience, China) in the supernatant were determined with ELISA kits.

Techniques: Western Blot, Expressing, In Vitro, Activity Assay, Bacteria, Enzyme-linked Immunosorbent Assay, Analysis

( A ) In vitro bactericidal activity of Kupffer cells against K. pneumoniae evaluated by enumerating viable bacteria; ( B–D ) IL-6 ( B ), IL-12 ( C ), and TNF-α ( D ) levels measured by ELISA; ( E-G ) Flow cytometry detection of IL-6 ( E ), IL-12 ( F ), and TNF-α ( G ) proportions; ( H ) Flow-cytometric analysis of Kupffer-cell phagocytic capacity; ( I ) Western blot detection of SHP2, p-SHP2, STAT1, p-STAT1, NF-κB p65, and p-NF-κB p65. * P < 0.05.

Journal: Journal of Microbiology and Biotechnology

Article Title: Anti-PD-1 Therapy Alleviates Klebsiella pneumoniae -Induced Liver Abscess by Effectively Activating Kupffer Cells

doi: 10.4014/jmb.2602.02040

Figure Lengend Snippet: ( A ) In vitro bactericidal activity of Kupffer cells against K. pneumoniae evaluated by enumerating viable bacteria; ( B–D ) IL-6 ( B ), IL-12 ( C ), and TNF-α ( D ) levels measured by ELISA; ( E-G ) Flow cytometry detection of IL-6 ( E ), IL-12 ( F ), and TNF-α ( G ) proportions; ( H ) Flow-cytometric analysis of Kupffer-cell phagocytic capacity; ( I ) Western blot detection of SHP2, p-SHP2, STAT1, p-STAT1, NF-κB p65, and p-NF-κB p65. * P < 0.05.

Article Snippet: The concentrations of TNF-α (E-EL-M3063, Elabscience, China), IL-6 (E-EL-M0044, Elabscience, China), and IL-12 (E-EL-M3062, Elabscience, China) in the supernatant were determined with ELISA kits.

Techniques: In Vitro, Activity Assay, Bacteria, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Analysis, Western Blot

ALOS ameliorates gut barrier function and promotes IL-22 production. A C-reactive protein (hs-CRP). B LPS. C Tumour necrosis factor-alpha (TNF-α). D Interleukin-6 (IL-6). E Interleukin 1β (IL-1β). F Interleukin 10 (IL-10). G Representative AB-PAS staining of ileum pathological sections (scale bar, 100 μm, n = 4). H Plasma IL-23. I Plasma IL-22. J Representative immunofluorescent staining of IL-22 in the ileum. n = 4 mice for each group. Scale bar, 20 μm. K Western blotting of the IL-22 and β-actin levels in the ileum tissue of ApoE −/− mice. *P < 0.05; **P < 0.01. One-way ANOVA (with Tukey's HSD) test in A–I. Unpaired t-test in J–K.

Journal: Natural Products and Bioprospecting

Article Title: Diversity of oligosaccharides in lipooligosaccharides of Akkermansia muciniphila and its anti-atherosclerotic activity

doi: 10.1007/s13659-026-00612-4

Figure Lengend Snippet: ALOS ameliorates gut barrier function and promotes IL-22 production. A C-reactive protein (hs-CRP). B LPS. C Tumour necrosis factor-alpha (TNF-α). D Interleukin-6 (IL-6). E Interleukin 1β (IL-1β). F Interleukin 10 (IL-10). G Representative AB-PAS staining of ileum pathological sections (scale bar, 100 μm, n = 4). H Plasma IL-23. I Plasma IL-22. J Representative immunofluorescent staining of IL-22 in the ileum. n = 4 mice for each group. Scale bar, 20 μm. K Western blotting of the IL-22 and β-actin levels in the ileum tissue of ApoE −/− mice. *P < 0.05; **P < 0.01. One-way ANOVA (with Tukey's HSD) test in A–I. Unpaired t-test in J–K.

Article Snippet: The interleukin-6 (IL-6) (SEKM-0007; Solarbio), tumor necrosis factor-alpha (TNF-α) (RK00027; Abclonal), interleukin-1β (IL-1β) (MM-0040M1; MEIMIAN), interleukin-10 (IL-10) (1418033–25-6; Solarbio), interleukin-22 (IL-22) (E-MSEL-M0040; Elabscience BiotechnologyCo., Ltd.), interleukin-23 (IL-23) (RK00017; Abclonal), oxidized LDL (ox-LDL), very low density lipoprotein cholesterol (VLDL) (ml037709-2; Mlbio), and lipopolysaccharide (LPS) (WLCSJZF202535; AMOY LUNCHANGSHUO) levels in the plasma and ileum were determined in commercial ELISA kits.

Techniques: Staining, Clinical Proteomics, Western Blot